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From sample to
immune insight

Ozette Lab

Ozette’s GCLP-compliant lab pairs custom and off-the-shelf high dimensional spectral cytometry assays with Ozette’s analytics platform to deliver the deepest characterization of the immune system.
  • High-parameter spectral analysis with the world’s largest commercially available flow cytometry panels.
  • Validated computational methods and best-in-class spectral unmixing ensure the highest quality data and comprehensive insights from precious clinical samples.
  • Expert team of computational biologists, immunologists, and translational scientists to support your unique project and get the most out of your high-dimensional data.
Talk to Our Lab team

Highest dimensional spectral cytometry assays.

Our validated 48-color spectral flow cytometry assay is purpose-built to characterize the complete circulating immune landscape from blood or PBMC samples. Out of the box, this assay covers functional, differentiation, and activation states across every major immune lineage with limitless marker tailoring to suit your unique scientific questions. This enables comprehensive immune profiling across innate and adaptive lineages with integrated functional state resolution.
Ozette's off-the-shelf 48-color panel:

Key Lineage: CD3, CD4, CD8, CD25, CD127, TCRγδ
Differentiation: CD45RA, CCR7, CD27, CD28, CD127, CD95
Activation: CD25, CD38, HLA-DR, ICOS
Exhaustion: PD1, TIGIT, CD39, BTLA
Trafficking: CCR4, CCR5, CCR6, CCR7, CXCR3, CXCR5

Key Lineage: CD19, CD20, CD27, IgD
Differentiation: IgM, IgG, CD24, CD38
Activation/Regulation: HLADR, BTLA, CD95
Trafficking: CCR5, CCR6, CXCR3, CXCR5

Key Lineage: CD2, CD16, CD56, CD127
NK Differentiation: CD57, KLRG1, CD27
NK Effector State: CD38, CD39, CD95
NK Activating/Cytotoxic Receptors: CD314, CD337
NK Regulatory Receptors: CD159a, CD159c, TIGIT
ILC Differentiation: CD45RA, CD117, TIGIT

Key Lineage: CD11c, CD14, CD16, HLADR, CD33, CD123
Monocyte Subsets: CD14, CD16
DC Subsets: CD11c, CD123, CD141, CD1c
Activation/Regulation: CD38, CD39, CD95, HLADR

Key Lineage: CD66b, CD123, HLADR (-)
Maturation: CD16, CD24, CD33
Activation: CD38, CD95

The depth of data generated by our 48-color panel is fully realized through Ozette’s computational analysis Platform to handle large and deep datasets. The assay paired with platform characterizes the full immune response and captures immunological insights that others miss.

Lab and analysis. One integrated workflow.

Ozette Lab data flows directly into our computational analysis platform. With 2⁴⁸ possible phenotypic combinations in a 48-color panel, the gap between conventional analysis and what’s actually in your data is significant. Leverage Ozette’s analytical platform to uncover the full biology of your samples.

  • Resolve Per-event spectral unmixing that adapts to every cell. Resolve eliminates artifacts, corrects for autofluorescence, and delivers cleaner data without FMOs.
  • Endpoints Computational gating that adapts to biological and temporal variability across samples, sites, and time to deliver biomarker endpoints you can trust.
  • Discovery Unbiased, AI-driven phenotype discovery that finds every robust immune population. Maximize your flow data to find rare and unexpected biological signal your standard gating template was never designed to see.
Learn more about our off the shelf 48-color panel and Ozette Discovery through our partnership with Bloodworks Bio
Bloodworks Bio Case Study

The only end-to-end solution for
deep immune profiling.

Obtaining reliable spectral flow data shouldn’t be a struggle. When you partner with Ozette Lab, you work directly with our expert teams to use our existing assays or design the right panel and analysis strategy for your program with data turnaround in weeks instead of months. Most clinical flow labs will tell you what you already know. Ozette is built to find what you didn’t know to look for.

1

Sample Receipt

Frozen PBMC, whole blood

2

Data Generation

Custom and off-the-shelf assays with full spectrum Cytek Aurora Instrumentation

3

ML-driven Computational Analysis

Resolve, Endpoints, and Discovery

4

Insights Delivery

Interactive data visualization, Bio-analytical reports, QC assured

Built by translational scientists to de-risk clinical trials

Our team brings deep expertise in high-dimensional immune profiling, clinical biomarker development, and cell therapy characterization. Leverage our 48-color off-the-shelf assay, or work with our expert team to design a high-parameter assay built around your biological questions.
Talk to Our Lab team