---

Upload Problems

• Issue: Files won’t upload
• Solution: Check file format (.fcs), size limits, internet connection

---

Gating Difficulties

• Issue: Can’t see clear populations
• Solution: Check control quality, adjust plot scales, try different detector combinations

---

Poor Unmixing Results

• Issue: Perceived spreading in results
• Solution: Review gate placement, check control file quality, consider different background sources

---

Control Set Creation Errors

• Issue: Missing required information
• Solution: Verify all metadata fields completed, check material type consistency

---

Getting Help

• Contact support@ozette.com for technical issues
• Check Appendix materials for additional guidance
• Consult with Ozette experts for experimental design

---

Error Messages

• “Panel name already exists”: Choose unique panel name
• “Missing required gates”: Ensure all three gate types are mapped
• “Upload failed”: Check file format and internet connection